Western blot was performed using Anti-GAP43 Polyclonal Antibody(Product # PA5-19470). Bands around 38-48 kDa band corresponding to GAP43 were observed across cell lines and tissues tested except Mouse lungs which is reported to show lower levels of GAP43. An additional uncharacterized band (*) around 50 kDa was also observed in all the models tested. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y differentiated to neurons (Lane 2), SK-N-SH (Lane 3), IMR-32 (Lane 4), Mouse Lung (Lane 5), Mouse Brain (Lane 6), Rat Brain (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).